Uptake and transport of zinc in the hyperaccumulator Thlaspi caerulescens and the non-hyperaccumulator Thlaspi ochroleucum
نویسندگان
چکیده
منابع مشابه
Transport and detoxification of cadmium, copper and zinc in the Cd/Zn hyperaccumulator plant Thlaspi caerulescens
Hyperaccumulators store most of the accumulated metal in the vacuoles of large leaf epidermal cells (storage cells). For investigating cadmium uptake, we incubated a protoplast mixture obtained by digestion of leaves of Thlaspi caerulescens (Ganges ecotype) with a Cd-specific fluorescent dye. A fluorescence kinetic microscope was used for selectively measuring Cd-uptake and photosynthesis in di...
متن کاملX-ray Imaging of Zinc Uptake and Accumulation in Hyperaccumulator Thlaspi caerulescens
The use of high energy x-ray fluorescence as a trace metal probe offers scientists an innovative tool for research. One application is the study of heavy metal accumulation in plant systems. The test species Thlaspi caerulescens has the ability to accumulate massive amounts of zinc, cadmium, and lead from contaminated soils as a form of bioremediation. In order to understand the efficiency of t...
متن کاملMolecular physiology of zinc transport in the Zn hyperaccumulator Thlaspi caerulescens.
In this manuscript, recent research from this laboratory into physiological and molecular aspects of heavy metal (Zn) transport in the hyperaccumulating plant species, Thlaspi caerulescens is reviewed. This research is aimed at elucidating the processes that underlie the accumulation of extraordinarily high levels of Zn in the T. caerulescens shoot (up to 3% Zn dry wt.) without any associated t...
متن کاملThe molecular physiology of heavy metal transport in the Zn/Cd hyperaccumulator Thlaspi caerulescens.
An integrated molecular and physiological investigation of the fundamental mechanisms of heavy metal accumulation was conducted in Thlaspi caerulescens, a Zn/Cd-hyperaccumulating plant species. A heavy metal transporter cDNA, ZNT1, was cloned from T. caerulescens through functional complementation in yeast and was shown to mediate high-affinity Zn(2+) uptake as well as low-affinity Cd(2+) uptak...
متن کاملCellular compartmentation of zinc in leaves of the hyperaccumulator thlaspi caerulescens
Cellular compartmentation of Zn in the leaves of the hyperaccumulator Thlaspi caerulescens was investigated using energy-dispersive x-ray microanalysis and single-cell sap extraction. Energy-dispersive x-ray microanalysis of frozen, hydrated leaf tissues showed greatly enhanced Zn accumulation in the epidermis compared with the mesophyll cells. The relative Zn concentration in the epidermal cel...
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ژورنال
عنوان ژورنال: Plant, Cell and Environment
سال: 1997
ISSN: 0140-7791,1365-3040
DOI: 10.1046/j.1365-3040.1997.d01-134.x